Fast Track Sequencing Services

Template in-Sequence out

Easy and quick sequencing of your
PCR products and plasmid

BAC Sequencing

Primer walking

This service including primer design and synthesis,
quality scoring and sequence assembly

Custom PCR & Sequencing

We provide services for custom PCR projects that include DNA dilution, PCR amplification, purification and fragment analysis or sequencing PCR primers.
We will also design and synthesize oligonucleotides for your custom PCR project needs.

Cloning & Sequencing

We offer cloning & sequencing for by cloning your DNA/PCR Products in a vector using standard cloning kits, followed by sequencing in ABi 3730Xl (Sanger sequencing methodology).

Microsatellite Selection & Analysis

AgriGenome labs is equipped with ABI 3730Xl machines with 36cm capillary array to facilitate the fragment analysis of your samples. Our machines are calibrated using Filter set G5 for dye set DS33 and can support 6-FAM, VIC, NED, PET and Liz as size std.

Custom Oligonucleotide Synthesis

We provide customized oligo nucleotides to cater your PCR, sequencing & genotyping needs. Oligos are made with high precision and delivered in a time bound manner.

Next-Gen Sequencing

Next-Generation Sequencing (NGS) involves high-throughput sequencing technologies that has reduced sequencing time and cost drastically. The availability of large volumes of data inexpensively has greatly impacted genetic, biological and medical research.

Sequencing technologies broadly follows the process of library preparation, sequencing and analysis. What differentiates the data output of one technology from the other is the combination of specific protocols that each one uses.

AgriGenome offers a comprehensive range of next generation sequencing services utilizing multiple sequencing platforms including Illumina HiSeq, MiSeq and Roche 454. Our team of wet lab scientists and bioinformaticians work with you to deliver reliable high quality results and provide end to end solutions for your NGS project needs.

Exome & Targeted Resequencing

Exome sequencing (also known as targeted exome capture) is a strategy to selectively sequence the coding regions of the genome and is a cheaper alternative to whole genome sequencing. In the human genome there are about 180,000 exons: these makes about 1% of the human genome, which translates to about 30 megabases (Mb) in length.

It is estimated that the protein coding regions of the human genome constitute about 85% of the disease-causing mutations. Exome sequencing is done to identify the functional variation that is responsible for both Mendelian and common diseases.

Enrichment methods available with us:

Platform : We offer exome sequencing on Illumina Hiseq.
For sales inquiries, please email us at: sales@aggenome.com

De Novo Genome Sequencing

De novo genome sequencing is used to characterize genomes where there is no reference sequence available, or in known genomes to study structural variation. De novo sequence reads are preferably generated on those NGS platforms that generate longer sequence reads like Roche 454 GS FLX Titanium or Pacbio SMRT, denovo sequencing can also be done on short read technolgies like Illumina HiSeq platforms with different insert sizes (Paired end and mate pair). Sequence reads from multiple platforms can also be combined for Hybrid assembly (e.g., Illumina + Roche 454) for cost effective coverage of large genomes, or with Sanger sequencing reads.

AgriGenome offers, Illumina Hiseq, Miseq, Roche 454 services and Sanger sequencing services for Denovo Genome sequencing and also offers bioinformatics data analysis for efficient assembly, gene prediction and annotation.

For sales inquiries, please email us at: sales@aggenome.com

Transcriptome ( RNA-Seq )

RNA-Seq is a powerful approach that enables researchers to discover, profile and quantify RNA transcripts in the whole transcriptome. As this method does not use predesigned probes or primers, it becomes an unbiased hypothesis free approach providing researchers with applications that are not possible by traditional microarray based methods.

RNA sequencing is used widely for:

Basis for expression quantification in RNA-Seq

1- Counting or Profiling
10 million total reads from poly-A selected RNA will give performance better than any microarray
2- Studying Alternative Splicing or quantifying cSNPs for most transcripts
Deeper profiling of 50 to 100 million reads, with read lengths of 50 to 100bps, from poly-A selected
3- Complete Annotation of an entirely New Transcriptome
~500 Million reads of 100 bp read length from multiple tissues

Platform : We offer Transcriptome sequencing on Illumina Hiseq platform
For sales inquiries, please email us at: sales@aggenome.com

Metagenomics

Metagenomics is the study of the variation of species in a complex microbial sample. Using NGS it is possible to study microbial communities by sequencing the gene of choice (e.g. 16S, V3, V4 regions) or the whole genome or transcriptome without the need for culturing.

Platform: Metagenomic sequence reads are generated on Illumina Miseq/ Hiseq or the Roche 454 GS FLX depending on the experiment objectives We can do the detailed analysis of the metagenomic NGS data.For sales inquiries, please email us at: sales@aggenome.com

Small RNA sequencing

Small RNA sequencing is a powerful application enabling the discovery and profiling of microRNAs and other non-coding RNA in any organism, without prior genome annotation. Using low RNA inputs, you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions.

With unprecedented sensitivity and dynamic range, small RNA sequencing methods allow for the most accurate detection and quantification of rare small RNA sequences.

Platform : We offer Transcriptome sequencing on Illumina Hiseq platform
For sales inquiries, please email us at: sales@aggenome.com

ChIP-seq (Chromatin Immunoprecipitation-Sequencing)

ChIP-seq is a technique or methodology to map transcription factor binding sites and histone modification status on a genome-wide scale. ChIP (Chromatin immunoprecipitation) comprises of a few basic steps: cross-linking a protein to chromatin, shearing the chromatin, using a specific antibody to precipitate the protein of interest with its associated DNA and reverse cross linking and finally purifying the associated DNA fragments.

AgriGenome accepts the ChIP DNA (200-800bp fragment size), then ChIP DNA libraries are made and run on Illumina Miseq or Hiseq and often generates tens to hundreds of millions of 50-100-bp sequences (also called short reads) from the 5' ends of ChIP-DNA fragments. We also do advanced data analysis of ChIP-seq data.

Platform: For sales inquiries, please email us at sales@aggenome.com

Genotyping & Microarrays

AgGenome Genotyping & Microarray Centre provdies end-to-end services using the following platforms:

Custom Cloning Service

Custom Cloning Service

The cloning team provides customized cloning and sub-cloning services in a preferred choice of vector, starting from DNA, RNA or from the gene sequence.

Options available at AgriGenome:

  • Cloning of PCR products Amplification of specific DNA fragments by PCR or gene- amplification using RT-PCR and cloning into a standard vector or into a vector of our choice
  • TA cloningCloning of PCR products into a TA cloning vector
  • ub-cloning of vector insertsSub-cloning of DNA fragments into target vectors, e.g. for in vivo protein expression, or for construction of hybrid genes
  • Cloning of synthetic genesDe novo synthesis of DNA fragments or complete genes and cloning into a standard vector or a vector of your choice, e.g. expression vectors.
  • Cloning genes from cDNA libraries into vectors of choice
  • Custom site-directed mutagenesisDesign and synthesis of mutagenesis primers, inverse PCR amplification and Sanger sequencing to confirm desired DNA mutations.

Custom protein expression, purification and functional validation service

The protein expression and purification services at AgriGenome provide a range of options to express and purify proteins of interest. Pure proteins are used in many applications in research and diagnostics.

We provide the following options:

  • Expression and purification of proteins from customer-supplied genes already cloned into an expression vector.
  • Sub-cloning of Ag protein gene fragment from cloning into expression vector and subsequent expression and affinity-purification of proteins.
  • Gene synthesis, cloning into expression vectors and production of purified protein.
  • Options to express proteins in prokaryotic expression system (E coli) and in mammalian expression systems according to customer needs.
  • Options of producing hard-to-express and toxic proteins in special bacterial strains.
  • Option for Ag protein expression in simple form or with fusion Tags to suit customer requirement.

Production of synthetic Fab and lgG for use as reagents

AgriGenome offers production of high-affinity monoclonal reagent-grade Fabs and full-length IgGs for use in basic and clinical research labs. It uses licensed proprietary phage display libraries to generate synthetic Fabs/IgGs. The diversity of peptides displayed in the library is >1010, which guarantees high affinity Fabs against antigens from any source.

Customer options available for getting Fab/IgG produced at AgriGenome include

  • Purified Ag protein (5mg/ml) can be supplied by customer against which Fab can be generated. Alternatively, customer can use our protein production pipeline to generate the antigen for screening.
  • Customer has the option to select Fabs or full-length IgGs, based on their requirement.
  • Option of getting the Ag-Fab or Ag-IgG validation assay optimizedis available with us
  • Customer can choose Fc region of IgG (human/mouse/rat/goat/others (specify), depending on their application.
  • Customer has the ability to opt for any special detection tags on the Fabs/IgGs.
  • AgriGenome Fabs/IgGs work best for FACS/IF/IP and ELISA and worth testing for WB and IHC.

We develop custom assays according to project needs.

  • ELISA kit assembly with pre-made Ag and Fab/IgG proteins and optimisation of assay parameters to cater customer requirement.
  • Customer can utilize AgriGenome Ag and Fab production pipeline to produce proteins and can get ELISA assay developed to suit application.
  • Diverse ELISA formats can be opted for kit assembly.
For further information on this service, or to discuss your project and get a customized quote for your requirement,
please email : sales@aggenome.com